Mesoporous titanium silicalite TS-1 with various crystallization time 2 4 and 8 days were successfully synthesized using microporous TS-1 as seeding material and cethyltrimethylammonium bromideCTABr as template. The solid products were characterized by X-ray Diffraction XRD Fourier Transform Infrared FTIR and Ultra Violet Visible Diffuse Reflectance UV-Vis DR spectroscopies N2 adsorption-desorption and Field Emission Scanning Electron Microscopy FESEM techniques to study structural properties of the samples. All of the catalyst material has MFI type of zeolite with orthorhombic structure. Diffractogram data stated that mesoporous TS-1 synthesized with crystallization time for 2 days had a regular mesopore which not observed for 4 and 8 days of crystallization time. Both of XRD pattern and FTIR spectra showed that the formation of mesophase decreased at longer crystallization time while the crystallinity was higher. UV-Vis DR spectra indicated that mesoporous TS-1 was arranged by tetrahedral titanium coordinated framework. It also suggested that there was a closed structure between mesoporous TS-1 and Ti-MCM-41 because of the presence of tetrahedral tripodal TiOSi3OH and tetrapodal TiOSi4 species. Variation of crystallization time had a great influence on the surface area and pore size of TS-1. All of the samples showed isotherm type IV of N2 adsorption-desorption. The BET mesopore surface area the mesopore volume and the pore size were decreased as crystallization time increased as followed 298 m2g 0.37 ccg and 381 nm 2 days 164 m2g 0.26 ccg and 3.42 nm 4days 141 m2g 0.17 ccg and 3.05 nm 8 days. This synthesized technique resulted TS-1 with combination of micro and meso phase that proved with t-plot data.