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ITS » Master Theses » Jaringan Cerdas Multimedia S2
Posted by at 23/12/2014 22:58:01  •  1956 Views



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Motilitas kelincahan gerak spermatozoa adalah salah satu faktor penentu kualitas sperma. Motilitas spermatozoa diketahui melalui uji mikroskopis sperma. Selama ini mikroskop laboratorium yang berharga mahal dan tidak portabel digunakan dalam perangkat uji sperma sebagai bagian dari CASA Computer Assisted Sperm Analyzer. Pada penelitian ini diajukan sperm analyzer system menggunakan mikroskop portabel pencahayaan LED yang berbasis pada metode iluminasi bright field. Pencahayaan ini memiliki kelebihan dibandingkan metode fase kontras dalam sistem CASA yaitu spektrum emisi LED tidak mengandung radiasi inframerah yang memperpendek waktu hidup spermatozoa. Sumber energi LED adalah baterai sehingga sperm analyzer system lebih mudah dibawa. Penjejakan spermatozoa dalam sperm analyzer system yang telah dikembangkan sebelumnya memiliki kelemahan yaitu hilangnya spermatozoa selama penjejakan karena bertabrakan dengan spermatozoa atau kotoran. Pada penelitian ini Mean Shift digunakan sebagai metode penjejak motilitas spermatozoa. Motilitas sperma diidentifikasi berdasarkan pada liniearitas lintasan dan kecepatan pergerakan LIN VCL dan VSL kedalam 4 class. Digunakan dua macam data yaitu data uji video motilitas spermatozoa koleksi UNSW Embryology dan data video hasil perekaman motilitas spermatozoa sapi menggunakan set up mikroskop yang diajukan. Hasil penjejakan delapan spermatozoa pada video koleksi UNSW Embryology adalah 625 class 1 125 class 2 125 class 3 dan 125 class 4. Hasil penjejakan spermatozoa sapi hasil rekaman adalah class 1.


Motility movement agility of sperm is one of factors in determining the quality of sperm. Spermatozoon motility is known through microscopic sperm test. Todays expensive unportable laboratory microscopes used as a sperm test device as a part of CASA Computer Assisted Sperm Analyzer. This study propose sperm analyzer system using a portable LED microscope based on bright field illumination method. This lighting has a better point compared to phase contrast methods in CASAs system that is LED emission spectrum contains no infrared radiation which would shorten the life time of spermatozoon. The energy source of LED are batteries so the sperm analyzer system is easy to carry. The tracking method used in the previous study has weakness which occur when the sperm movement being investigate collide with other sperm or a debris. In this study Mean Shift is used as the tracking method of the spermatozoon movement. Sperm motility is identified based on linearity and movement speed LIN VCL and VSL into 4 classes. Two kinds of data were used spermatozoon motility video test belong to the collection of UNSW Embryology and cattle sperm video data recorded using the proposed microscope set up. The tracking result of eight spermatozoon for UNSW Embryology video collection were 62.5 of class 1 12.5 of class 2 12.5 of class 3 and 125 of class 4. The results of tracking cattle spermatozoon recordings were of class 1.

Keywordscairan semen; penjejakan spermatozoa; Mean Shift; VSL; LIN
Subject:  Algoritma komputer
  1. Dr. I Ketut Eddy Purnama, ST., MT.
  2. Muhtadin, ST., MT.
Date Create: 08/01/2013
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Master-22103140001153
Collection ID: 22103140001153
Call Number: RTE 005.1 Ago

Master Theses of Electrical Engineering, RTE 005.1 Ago i, 2014

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Copyright @2014 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library

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  1.  ITS-Master-35051-2210205201-Abstract_id.pdf - 184 KB
  2.  ITS-Master-35051-2210205201-Abstract_en.pdf - 184 KB
  3.  ITS-Master-35051-2210205201-Conclusion.pdf - 195 KB

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