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ITS » Master Theses » Teknik Manajemen Lingkungan S2
Posted by tondoindra@gmail.com at 27/03/2015 18:32:03  •  802 Views


HIDROLISIS EICHHORNIA CRASSIPES MENGGUNAKAN PROSES FISIKA KIMIA DAN BIOLOGIS PADA TAHAP AWAL PEMBUATAN BIOETANOL

HYDROLYSIS OF Eichhornia crassipes USED PHYSICAL CHEMICAL AND BIOLOGICAL PROCESS IN FIRST PHASE BIOETHANOL RODUCTION

Author :
HANDAYANI, ANNISA ( 3312201008 )




ABSTRAK

Kelimpahan Eichhornia crassipes eceng gondok sangat merugikan ekosistem air namun berpotensi menghasilkan bioetanol bila diproses. Trichoderma viride agen pendegradasi lignin dan Phanaerochaete chrysosporium agen pendegradasi hemiselulosa dan selulosa dengan baik. Asam akan memaksimalkan proses degradasi tersebut pada tahap hidrolisis. Kadar glukosa hasil hidrolisis sangat menentukan banyaknya bioetanol yang dihasilkan pada proses selanjutnya. Oleh karena itu tujuan penelitian ini adalah menganalisis dan mengevaluasi kondisi hidrolisis E. crassipes menggunakan proses fisika kimia dan biologis yang optimum pada tahap awal pembuatan bioetanol. Metode yang dilakukan adalah eksperimen laboratorium dengan persiapan bahan baku biakan kapang T. viride dan P. chrysosporium pretreatment menggunakan P. chrysosporium selama enam hari kemudian hidrolisis. Treatment kombinasi hidrolisis dilakukan selama 1 2 3 4 dan 5 hari pada suhu ruang juga 400C terhadap kapang. Selain itu juga dilakukan hidrolisis asam menggunakan H2SO4 025 dan 05. Parameter kadar glukosa masing-masing diukur menggunakan Metode Nelson-Somogyi. Standart deviasi ditentukan untuk mengetahui perbedaan nyata antar grafik yang dibentuk oleh setiap kombinasi hidrolisis. Produksi glukosa kombinasi hidrolisis Eichhornia crassipes menggunakan fisik kimia dan biologi yang optimum adalah selama empat hari. Kombinasi hidrolisis H2SO4 025 yang optimum adalah pada suhu ruang dengan produksi glukosa 979 mgg 154 mgg oleh aktivitas T. viride dan 782 mgg 111 mgg oleh aktivitas P. chrysosporium. Kombinasi hidrolisis H2SO4 05 yang optimum adalah pada suhu 400C dengan produksi glukosa 724 mgg 91 mgg oleh aktivitas T. viride dan 86 mgg 126 mgg oleh aktivitas P. chrysosporium. Produksi glukosa naik sampai hari keempat dan turun pada hari kelima karena pengaruh keterbatasan sumber makanan bagi kapang dalam substrat.


ABSTRACT

Abundance of Eichhornia crassipes water hyacinth results in impaired water ecosystem but potentially produce bioethanol. Trichoderma viride is a good lignin degradation agent and Phanaerochaete chrysosporium is a hemicellulose and cellulose degradation agent. Acid addition is an effective way to increase the hydrolysis efficiency. Glucose that produced by hydrolysis determine the amount of bioethanol in the next processes. The purpose of this research was to analyze and evaluate the optimum hydrolysis condition of E. crassipes by means of physical chemical and biological processes in first phase of bioethanol production. This research is a laboratory-based experiment which include raw material preparation T. viride and P. chrysosporium culture biological pretreatment with P. chrysosporium forsix days prior to hydrolysis. Hydrolysis combination was done for 1 2 3 4 and 5 days in room temperature and 400C. These temperatures was applied regarding the fungus conditions. Moreover treatment was done using H2SO4 025 and 05. Parameter of glucose was calculated using Nelson- Somogyi Method. The optimum combination of hydrolysis Eichhornia crassipes by means of physical chemical and biological processes produced glucose for four days. The optimum hydrolysis combination used H2SO4 025 was in the room temperature. These combination produced glucose amount 979 mgg 154 mgg by T. viride activity and amount 782 mgg 111 mgg by P. chrysosporium activity. The optimum hydrolysis combination used H2SO4 05 was in the 400C. These combination produced glucose amount 724 mgg 91 mgg by T. viride activity and 86 mgg 126 mgg by P. chrysosporium activity. Glucose production was increased till fourth days and decreased on fifth days because influence of limitedness of food source for fungi.



KeywordsE.crassipes, hidrolisis, fisika, kimia, biologis.
 
Subject:  Limbah; Pembuangan; fenol
Contributor
  1. Dr. Ir. Ellina S. Pandebesie, M.T.
Date Create: 30/01/2014
Type: Text
Format: PDF
Language: Indonesian
Identifier: ITS-Master-33103150001234
Collection ID: 33103150001234
Call Number: RTL 628.3 Han h


Source
Master Theses Of Environment Engineeing RTL 628.3 Han h, 2014

Coverage
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Rights
Copyright @2015 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




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  1.  ITS-Master-36605-3312201008-abstract_id.pdf - 708 KB
  2.  ITS-Master-36605-3312201008-abstract_en.pdf - 707 KB
  3.  ITS-Master-36605-3312201008-conclusion.pdf - 369 KB




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