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ITS » PhD Theses » Program Doktoral Teknik Kimia
Posted by tondoindra@gmail.com at 26/09/2014 17:17:53  •  1066 Views


BIOKONVERSI ONGGOK MENJADI ETHANOL DENGAN MENGGUNAKAN MULTIENZIM

BIOCONVERSION OF CASSAVA PULP INTO ETHANOL BY USING MULTIENZIME

Author :
DJUMA`ALI ( 2306301801 )




ABSTRAK

Onggok limbah padat pabrik tapioka yang murah diusulkan sebagai substrat fermentasi ethanol yang sangat berpotensi karena kandungan patinya masih tinggi 655 dan serat 1312 kadar abunya rendah dan ukuran partikel serat lignoselulosanya kecil. Kelayakan ekonomi proses ditentukan oleh kesempurnaan degradasi polisakaridanya menjadi glukosa potensi hidrolisis komparatif dari enam enzim komersial seprti Cellobiase Dextrozyme GA Termamyl 120 L Celluclast 1.5 L Viscozyme L dan Pectinex Ultra SP-L perlu dipelajari. Tiga enzim yang disebut pertama merupakan enzim amilolitik Celluclast 1.5 L memiliki aktivitas utama sebagai enzim selulolitik dan Viscozyme L serta Pectinex Ultra SP-L keduanya aktif sebagai enzim pektinolitik. Dextrozyme GA 563 mLkg substrat 50C pH 5 24 jam sangat efektif menghidrolisa onggok 12 bv yang telah diberi perlakuan hidrothermal 90C 10min dan sekitar 689 g glukosa dibebaskan per kg onggok atau setara 9018 DE Dextrose Equivalent . Maserasi terhadap onggok dengan konsentrasi lebih tinggi 23 bv ukuran partikel 177956m dengan Celluclast 1.5 L 167 mLkg substrat 50C pH 5 180 menit kemudian dilikuifikasi dan disakarifikasi masing-masing dengan Termamyl 120 L 25 mLkg substrat 90C pH 5 30 menit dan Dextrozyme GA 563 mLkg substrat 50C pH 5 24 jam menghasilkan glukosa 738gkg onggok atau setara 966 DE. Multi enzim kasar Viscozyme L 858 mLkg substrat Cellobiase 563 mLkg substrat pada 50C 24jam pH 5 yang terdiri dari enzim penghidrolisis polisakarida non pati seperti selulase pektinase dan hemiselulase bekerja sama membebaskan ganula pati yang terperangkap dalam struktur serat dinding sel onggok sebelum disakarifikasi oleh aktivitas pengurai pati mentah. Fermentable sugars yang dihasilkan sebesar 535 gram per kg onggok DE 701. Berdasarkan hasil percobaan diperoleh neraca konversi bahan untuk menghasilkan 10 kg ethanol dibutuhkan 557 kg onggok berkadar air 40 dengan perlakuan hidrothermal 90C dan untuk onggok kering berkadar air 15 diperlukan 393 kg


ABSTRACT

Cassava pulp a low cost solid byproduct of the cassava starch industry has been proposed as a high potential ethanolic fermentation substrate due to its high unextracted starch level 65.5 and 13.12 fiber residual low ash content and tiny particle size of the lignocellulosic fibers. As the economic feasibility of its process depends on complete degradation of the polysaccharides to fermentable glucose the comparative hydrolytic potential of six commercial enzymes such as Cellobiase Dextrozyme GA Termamyl 120 L Celluclast 1.5 L Viscozyme L and Pectinex Ultra SP-L was studied. The three earlier mentioned enzymes were expressed as an amylolytic enzyme Celluclast 1.5 L had major activity as cellulolytic enzyme and both Viscozyme L and Pectinex Ultra SP-L had main activity as pectinolytic enzyme. Dextrozyme GA 5.63 mLkg substrate 50C pH 5 24h showed the most effective activity on hydrothermal 90C 10min treated cassava pulp 12 dw v and approximately 689 g glucose was released per kg cassava pulp or equivalent to 9018 dextrose equivalent DE. Maceration of higher concentration 23 dwv particle size 177956m of cassava pulp by Celluclast 1.5 L 16.7 mLkg substrate 50C pH 5 180min then liquefied and saccharified by Termamyl 120 L 25 mLkg substrate 90C pH 5 30min and Dextrozyme GA 5.63 mLkg substrate 50C pH 5 24h respectively resulted in a glucose yield of 738gkg cassava pulp or equivalent to 96.6 DE. Crude multi-enzyme Viscozyme L 858 mLkg substrate Cellobiase 5.63 mLkg substrate 50C 24h pH. 5 composed of non-starch polysaccharide hydrolyzing enzyme activities including cellulase pectinase and hemicellulase act cooperatively to release the trapped starch granules from fibrous cell wall structure for subsequent saccharification by raw starch degrading activity. A high yield of fermentable sugars 535 gkg cassava pulp released or equivalent to 70.1 DE. Based on the experiment result the need of cassava pulp for ethanol production can be estimated. For instance 5.57kg of 40 water content cassava pulp was needed for production of 1.0 kg ethanol. With the same condition 3.93kg dry cassava pulp 15 water content was needed to produce 1 kg ethanol.



Keywordsbiokonversi, multi-enzim, onggok, DE, hidrothermal
 
Subject:  Fermentasi;Hidrolisis
Contributor
  1. Prof.Dr.Ir. Nonot Soewarno M.Eng
  2. Dr.Ir. Soemarno M.Eng
Date Create: 22/03/2013
Type: Text
Format: PDF
Language: Indonesian
Identifier: ITS-PhD-23004140000095
Collection ID: 23004140000095
Call Number: RDK 660.284 49 Dju b


Source
PhdTheses of Chemical Engineering RDK 660.284 49 Dju b,2014

Coverage
ITS Community

Rights
Copyright @2013 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




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  1.  ITS-PhD-31867-2306301801-Abstract_id.pdf - 207 KB
  2.  ITS-PhD-31867-2306301801-Abstract_en.pdf - 207 KB
  3.  ITS-PhD-31867-2306301801-Conclusion.pdf - 338 KB




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