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ITS » Undergraduate Theses » Biologi Posted by aprill@is.its.ac.id at 17/02/2016 13:59:44 • 816 Views
PENGARUH MEDIUM LIMBAH ORGANIK TERHADAP AKTIVITAS ENZIM PROTEASE DARI ISOLAT KAPANG TANAH WONOREJO
THE INFLUENCE OF ORGANIC WASTE MEDIUM AGAINST THE ACTIVITY OF PROTEASE ENZYME FROM WON0REJO MOLDS ISOLATE
Author : DEWI, NI WAYAN NANIK SANTIKA ( 1510100021 )
ABSTRAK
Meningkatnya akumulasi limbah cair organik yang mengandung protein di lingkungan perlu ditanggulangi. Penelitian ini bertujuan untuk mengetahui aktivitas enzim protease dari Penicillium sp. 3 T3F2 Verticillium sp. T3.3 dan Trichoderma sp. 1 T3I2 secara tunggal dan konsorsium pada medium limbah cair organik. Parameter yang diuji dalam penelitian ini adalah aktivitas enzim protease dan degradasi protein isolat pada medium limbah cair pencucian ikan limbah tangki septik dan limbah cair tahu. Data pengaruh medium terhadap aktivitas enzim protease dianalisis menggunakan Annova One-Way dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa isolat berpengaruh terhadap aktivitas enzim protease dari ketiga medium. Konsorsium isolat Penicillium sp. 3 T3F2 Verticillium sp. T3.3 memiliki nilai aktivitas enzim protease dan degradasi protein tertinggi pada ketiga jenis medium. Pada medium limbah cair
pencucian ikan 0.219 UmL 48.394 . Pada medium limbah tangki septik 0.149 UmL 31.268 . Pada limbah cair tahu
0.222 UmL 44.627 .
ABSTRACT
Increasing the accumulation of organic wastewater containing proteins in the neighborhood need to overcome. This
research is to know the activity of an enzyme protease from Penicillium sp. 3 T3F2 Verticillium sp. T3.3 and Trichoderma sp. 1 T3I2 single and a consortium on substrate organic wastewater. The parameters tested in this research is activity of an
enzyme protease and degradation of protein by isolates on substrate fish processing wastewater septic tank wastewater and tofu wastewater. The influence data of substrate against the
activity of an enzyme protease analyzed using Annova One-Way and continued with Duncan test. The result showed that substrate and isolates affect the activity of enzymes protease. A consortium of isolates Penicillium sp. 3 T3F2 Verticillium sp.T3.3 having value activity of an enzymes protease and degradation of the protein highest in the three types of substrate. In substrate fish processing wastewater 0.219 UmL 48.394 . In Septic tank wastewater 0.149 UmL 31.268 . In tofu wastewater 0.222 UmL 44.627.
Keywords:
Limbah Organik; Protease; Degradasi Protein
Subject
: Lipase - bioteknologi
Contributor
Nengah Dwianita Kuswytasari, S.Si.,M.Si
Date Create
: 17/02/2016
Type
: Text
Format
: pdf
Language
: Indonesian
Identifier
: ITS-Undergraduate-15001150008307
Collection ID
: 15001150008307
Call Number
: RSBi 572.76 Dew p
Source Undergraduate Theses of Biology, RSBi 572.76 Dew p, 2016
Coverage ITS Community
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