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ITS » Undergraduate Theses » Biologi
Posted by at 10/03/2016 15:34:16  •  600 Views



Author :
KHOTIMAH, KUSNUL ( 1510 100 043 )


Merkuri merupakan logam berat paling toksik dibandingkan dengan logam berat lainnya. Beberapa bakteri ada yang resisten merkuri. Salah satu genus bakteri resisten merkuri dan mampu mengakumulasi merkuri yaitu Azotobacter. Azotobacter merupakan bakteri pemfiksasi nitrogen bebas non simbiotik yang melimpah di daerah rhizosfer lahan pertanian dan merupakan bakteri penghasil EPS yang dapat berfungsi sebagai pengkhelat logam. Penelitian ini bertujuan untuk mendapatkan isolat Azotobacter yang resisten terhadap merkuri HgCl2 dan mengukur kemampuan bioakumulasinya terhadap HgCl2. Isolasi bakteri Azotobacter dilakukan dengan media selektif Azotobacter. uji resistensi HgCl2 dilakukan dengan streak agar miring dan kemampuan bioakumulasi diukur dengan metode serapan atom serta uji viabilitas menggunakan metode pour plate. Analisis beda nyata dengan ANOVA pada taraf 5 dilanjutkan dengan uji beda nyata terkecil BNT. Tiga isolat Azotobacter dari lahan eco urban farming ITS resisten terhadap HgCl2 sampai 20 mgL yaitu A5 A6 dan A9. Efisiensi bioakumulasi yang tertinggi pada pemaparan HgCl2 5 mgL yaitu isolat A5 89 dan A9 87.


Mercury is the most toxic heavy metals compared with other heavy metals. Some bacteria are resistant to mercury. One of mercury resistant bacteria genus and able to accumulate of mercury is Azotobacter. Azotobacter is free-living nitrogen fixing bacteria which abundant in rhizosfer area of agricultural land and it is EPS producing bacteria that can serve as chelating metals. This research aims to get Azotobacter isolates resistant to mercury HgCl2 and measure the ability of mercury biaccumulation. Isolation of Azotobacter by selective Azotobacter media resistance test by streak at slant agar and bioaccumulation ability measured by atomic absorption method and vaibility test using pour plate method. Observed data analyzed by ANOVA continued by Least Significant Difference LSD test Both test at level 5 . Three Azotobacter isolates from eco urban farming ITS land are resistant until 20 mgL of HgCl2 which is A5 A6 and A9. The highest efficiency bioaccumlation on exposure 5 mgL HgCl2 is A5 89 and A9 87.

KeywordsAzotobacter; bioakumulator; merkuri; uji resistensi
Subject:  Air raksa
  1. Dr. Enny Zulaika, M.P
Date Create: 10/03/2016
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-15001150008397
Collection ID: 15001150008397
Call Number: RSBi 571.954 663 Kho a

Undergraduate Theses of Biology, RSBi 571.954 663 Kho a, 2016

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Copyright @2016 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library

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  1.  ITS-Undergraduate-40677-1510100043-Abstract_id.pdf - 197 KB
  2.  ITS-Undergraduate-40677-1510100043-Abstract_en.pdf - 197 KB
  3.  ITS-Undergraduate-40677-1510100043-Conclusion.pdf - 198 KB

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