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ITS » Undergraduate Theses » Biologi Posted by aprill@is.its.ac.id at 11/03/2016 09:00:00 • 1073 Views
AKTIVITAS ENZIM MERKURI REDUKTASE PADA AZOTOBACTER SEBAGAI ENZIM PEREDUKSI HG2 MENJADI HG0
MERCURY REDUCTASE ACTIVITY OF Azotobacter ISOLATES AS A REDUCING AGENT FOR MERCURIAL ION Hg2
Author : SAKINAH, ANJAR LULU ( 1510 100 046 )
ABSTRAK
Merkuri adalah logam berat yang sangat tosik dan berbahaya. Beberapa bakteri dapat hidup di habitat yang tercemar merkuri disebut bakteri resisten merkuri satu diantaranya adalah Azotobacter. Tujuan penelitian adalah mendapatkan isolat unggul Azotobacter dari lahan Eco Urban Farming ITS yang resisten terhadap HgCl2 dan mengetahui kemampuan aktivitas enzim merkuri reduktase sebagai bioreduktor Hg2 menjadi Hg0. Isolasi dilakukan menggunakan media selektif Azotobacter agar. Pengujian resistensi merkuri dilakukan dengan metode goresan
agar miring yang megandung HgCl2. Aktivitas enzim merkuri reduktase dianalisis dengan metode MRA dan spektofotometri pada panjang gelombang 340 nm. Hasil isolasi mendapakan 10 isolat Azotobacter resisten HgCl2 isolat A5 dan A9 mempunyai resistensi 10 mgL HgCl2 dengan pertumbuhan yang optimum dan mempunyai aktivitas
merkuri reduktase relatif tinggi dengan daya reduksi 3 mgL dan efisiensi reduksi 70 pada konsentrasi 5 mgL HgCl2.
ABSTRACT
Mercury is a heavy metal that its dangerous toxic. Some bacteria can living at contaminated mercury areas called mercury resistant bacteria which one of them Azotobacter. The purpose of this researched to get Azotobacter from Eco Urban Farming ITS land which resistant mercury HgCl2 and determine activity of mercury reductase enzyme for reduction Hg2 to Hg0. Methods isolation used selective Azotobacter agar mercury resistant with added HgCl2 different concentration and activity of mercury reductase enzyme analyzed MRA method and spechtophotometry at 340 nm. The result of isolation got 10 isolate Azotobacter what resistant mercury HgCl2 isolate A5 and A9 resistant 10 mgL. with optimum growth and they have activity of mercury reductase enzyme more higher with reduction abilities 3 mgL and
efficiency reduction Hg2 70 in HgCl2 5 mgL concentration.
Keywords:
Azotobacter; enzim merkuri reduktase; gen mer operon; Merkuri HgCl2
Subject
: Mercury - analisis; Pengurangan (kimia)
Contributor
Dr. Enny Zulaika, M.P.
Date Create
: 11/03/2016
Type
: Text
Format
: pdf
Language
: Indonesian
Identifier
: ITS-Undergraduate-15001150008399
Collection ID
: 15001150008399
Call Number
: RSBi 571.954 663 Sak a
Source Undergraduate Theses of Biology, RSBi 571.954 663 Sak a, 2016
Coverage ITS Community
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