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ITS » Undergraduate Theses » Biologi
Posted by fandikaaqsa@its.ac.id at 28/04/2016 09:02:22  •  921 Views


PENGARUH MEDIUM LIMBAH ORGANIK TERHADAP AKTIVITAS ENZIM LIPASE DARI ISOLAT KAPANG TANAH WONOREJO

THE INFLUENCE OF ORGANIC WASTE SUBSTRATE AGAINST THE ACTIVITY OF LYPASE ENZYME FROM WON0REJO MOLDS ISOLATE

Author :
KURNIAWATI, ALFIA RAHMA ( 1510100020 )




ABSTRAK

Meningkatnya akumulasi limbah cair organik yang mengandung lipid di lingkungan perlu ditanggulangi. Penelitian ini bertujuan untuk mengetahui aktivitas enzim yang dihasilkan dari isolat kapang Mortierella sp. T3.G1 Aspergillus niger T2.1 Penicillium sp.1 T4.E3 secara tunggal dan konsorsium dalam mendegradasi lipid pada medium limbah organik yang berbeda. Parameter yang diamati dalam penelitian ini adalah nilai degradasi lipid dan nilai aktivitas enzim lipase. Limbah cair organik yang digunakan adalah limbah cair pencucian ikan limbah cair tangki septik dan limbah minyak goreng bekas. Data pengaruh medium limbah cair organik terhadap aktivitas enzim lipase dianalisis dengan menggunakan ANOVA oneway dan dilanjutkan dengan uji Duncan. Hasil menunjukkan bahwa medium dan isolat berpengaruh terhadap aktivitas lipase dengan nilai P0000. Korelasi nilai aktivitas dan nilai degradasi tertinggi ditunjukkan pada penambahan isolat Aspergillus niger dengan nilai aktivitas lipase sebesar 01511 UmL dan degradasi lipid 8395 pada limbah minyak goreng bekas 00997 UmL dan degradasi lipid 5843 pada limbah pencucian ikan dan aktivitas lipase 006


ABSTRACT

Increasing the accumulation of organic waste liquid that containing lipids in the neighborhood need to overcome. This research is aimed to know the activity of an enzyme lipase from mold isolate Mortierella sp. T3.G1 Aspergillus niger T2.1 and Penicillium sp.1 T4.E3 singly and a consortium on different medium liquid waste organic. The parameters that are tested in this research is the degradation of lipid and the activity of an enzyme lipase. Organic waste liquid that used there are processing fish wastewaterseptic tank wastewater and used cooking waste oil. The influence data of medium against the activity of an enzyme protease analyzed using Annova One-Way and continued with Duncan test. The result showed that medium and isolates affecting the activity of enzymes lipase with P value0000. Correlation of activity value and degradation value indicated that isolate Aspergillus niger is the highest in three organic waste liquid with activity lipase 00997 UmL and degradation lipid 5843 on processing fish wastewater activity lipase 01511 UmL and degradation lipid 8395 on used cooking waste oil and lipase 00697 UmL and degradation lipid 6736 on septic tank wastewater.



KeywordsLimbah Organik; Lipase; Degradasi Lipid
 
Subject:  Bioteknologi
Contributor
  1. Nengah Dwianita Kuswytasari, S.Si.,M.Si
Date Create: 28/04/2016
Type: Text
Format: PDF
Language: Indonesian
Identifier: ITS-Undergraduate-15001160008662
Collection ID: 15001160008662
Call Number: RSBi 660.634 Kur p


Source
Undergraduate Thesis of Biology, RSBi 660.634 Kur p, 2014

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  1.  ITS-Undergraduate-41375-1510100020-abstract_id.pdf - 197 KB
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