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ITS » Undergraduate Theses » Biologi
Posted by anisw@its.ac.id at 28/04/2016 09:33:50  •  523 Views


BIOGROUTING PRODUKSI UREASE DARI BAKTERI LAUT OCEANOBACILLUS SP. PENGENDAP KARBONAT

BIOGROUTING UREASE PRODUCTION FROM CARBONAT PRESIPITATION BACTERIA Oceanobacillus sp.

Author :
AINIYAH, SIDRATU ( 1510100010 )




ABSTRAK

Biogrouting adalah teknologi yang mensimulasikan proses diagenesis yaitu transformasi butiran pasir menjadi batuan pasir calcarinitesandstone. Permasalahan dalam penelitian ini adalah bagaimana mengoptimasi produk urease dengan melakukan uji aktifitas mengisolasi mempurifikasi dan mengkarakterisasi urease serta mengaplikasikannya sebagai material grout. Uji aktifitas dan optimasi dilakukan dengan menumbuhkan isolat Oceanobacillus sp. pada 2 variasi medium B4 urea dan B4 urin 5 variasi pH 4-8 dan 2 variasi suhu 25C dan 29C. Hasil uji aktifitas dan optimasi selanjutnya dipurifikasi menggunakan ammonium sulfat Uji Bradford dan dicari titik isoelektriknya. Kemudian hasil protein presipitat dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil penelitian diketahui bahwa aktifitas urease paling tinggi adalah 70.21 unitml. Urease optimal dihasilkan pada isolat yang ditumbuhkan pada B4 urea pada pH 7 temperatur 25C. Berat molekul urease yang dikarakterisasi menggunakan SDS-PAGE adalah 440 kDa sedangkan titik isoelektriknya pada pH 6. Urease dapat dijadikan material grout karena memiliki kemampuan untuk melakukan sementasi pada aplikasi sederhana biogrouting.


ABSTRACT

Grouting is the process of pore filling with grout material construction material. Biogrouting is a technology that simulates the process of diagenesis namely the transformation of grains of sand into sandstone calcarinite sandstone. Calcite CaCO 8323 formed from biogrouting process would bind sand grains causing the cementation process. The research problem is how to optimize the product with the urease activity test isolate purify and characterize urease and applying it as a grout material. Testing and optimization activities carried out by growing isolates Oceanobacillus sp. 2 variations on the medium urea and B4 B4 urine 5 variations of pH 4-8 and 2 variations of temperature 25 C and 29 C. Test results and optimization activities further purified using ammonium sulfate Test Bradford and sought isoelectric point. Then the results of the protein precipitates were characterized using SDS-PAGE. Based on the survey results revealed that the highest urease activity was 70.21 units ml generated from isolates of urease on urea medium B4. Optimal urease produced in isolates grown in medium B4 urine pH 8 and a temperature of 25 C while in medium B4 urea at pH 7 a temperature of 25 C. The molecular weight of urease were characterized using SDS-PAGE was 440 kDa whereas the isoelectric point at pH 6. Urease can be used as grouting material because it has the ability to do a simple application biogrouting cementation.



KeywordsBiogrouting; Diagenesis; Oceanobacillus sp.; Urease
 
Subject:  NONE
Contributor
  1. Dr. techn. Endry Nugroho Prasetyo, MT
Date Create: 28/04/2016
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-15001160008734
Collection ID: 15001160008734
Call Number: RSBi 552.58 Ain b


Source
Undergraduate Theses of Biology, RSBi 552.58 Ain b, 2014

Coverage
ITS Community

Rights
Copyright @2016 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




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  1.  ITS-Undergraduate-41381-1510100010-Abstract_id.pdf - 252 KB
  2.  ITS-Undergraduate-41381-1510100010-Abstract_en.pdf - 282 KB
  3.  ITS-Undergraduate-41381-1510100010-Conclusion.pdf - 306 KB




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