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ITS » Undergraduate Theses » S1 Teknik Kimia - Lintas Jalur
Posted by dee@its.ac.id at 05/01/2015 17:32:52  •  1194 Views


PRODUKSI GULA REDUKSI DARI SABUT KELAPA DENGAN HIDROLISIS ENZIM SELULASE DAN ENZIM XILANASE MENGGUNAKAN PRETREATMENT NAOH DAN LARUTAN IONIC LIQUID

PRODUCTION OF REDUCING SUGAR FROM COCONUT COIR USING HYDROLYSIS BY CELLULASE ENZYME AND XYLANASE ENZYME WITH PRETREATMENT NAOH AND IONIC LIQUID SOLUTION

Author :
DEWI, HELLEN KARTIKA ( 2311106005 )
PUSPASARI, DEBRA ARLIN




ABSTRAK

Dunia sedang menghadapi problem penggunaan energi berbasis fosil seperti minyak bumi dan gas alam yang semakin langka karena tidak dapat diperbarui. Oleh karena itu penemuan sumber energi dari bahan yang dapat diperbarui sangat dibutuhkan untuk memenuhi kebutuhan energi dunia yang semakin lama semakin meningkat.Bahan baku yang digunakan untuk menghasilkan gula reduksi adalah sabut kelapa. Komposisi Kimia sabut kelapa terdiri dari 27 selulosa 18 hemiselulosa 41 lignin 2 abu dan 13 extrak.Tujuan dari penelitian ini adalah untuk mendapatkan metode terbaik dalam pretreatment sabut kelapa yaitu dengan variabel larutan NaOH 1 dan NaOH 4 IL Ionic Liquid atau larutan NaOH lalu dilanjutkan dengan IL. Metode penelitian yang digunakan terdiri dari 2 tahap yaitu tahap pretreatment bahan baku baik mekanik maupun kimia dan tahap hidrolisis sabut kelapa. Pada tahap pretreatment secara mekanik sabut kelapa digiling dan diayak untuk mendapatkan ukuran 100-120 mesh untuk pretreatment secara kimia digunakan NaOH dengan konsentrasi 1 dan 4 dengan suhu 80 oC dan waktu pretreatment selama 16 jam lalu dilanjutkan dengan pretreatment dengan larutan ionic liquid pada suhu 120 C selama 15 jam. Enzim yang digunakan adalah enzim selulase murni dari strain Aspergillus niger dan enzim xilanase murni dari strain Trichoderma longibrachiatum yang telah diencerkan sebesar 18.6 Ugram sabut kelapa. Hidrolisa dilakukan pada suhu 60 C dan pH 3 selama 48 jam. Setelah dilakukan pretreatment kandungan selulosa tertinggi adalah 4333 hasil pretreatement sabut kelapa dengan NaOH 1 dilanjutkan dengan pretreatment IL. Kandungan Hemiselulosa tertinggi adalah 2518 dari sabut kelapa yang dilakukan pretreatement dengan IL dan lignin terendah diperoleh dari sabut kelapa hasil pretreatment NaOH 1 yaitu sebesar 2522. Selulosa dan hemiselulosa hasil pretreatment dengan HaOH 1 lebih baik daripada pretreatment NaOH 4 sebesar 4318 dan 2057. Hidrolisis menggunakan enzim campuran selulase dan xilanase menghasilkan gula reduksi paling tinggi sebesar 3139 sedangkan hidrolisis menggunakan enzim selulase yield tertinggi 2705 gram gula reduksigramselulosa hemiselulosa awal. Hidrolisis menggunakan enzim campuran selulase dan xilanase menghasilkan gula reduksi untuk tanpa pretreatment pretreatment NaOH 1 pretreatment NaOH 4 Pretreatment IL dan pretreatment NaOH 1 dilanjutkan IL adalah sebesar 272 gL 3834 gL 3475 gL 3168 gL dan 4645 gL. Hidrolisis menggunakan enzim selulase menghasilkan gula reduksi untuk tanpa pretreatment pretreatment NaOH 1 pretreatment NaOH 4 Pretreatment IL dan pretreatment NaOH 1 dilanjutkan IL adalah sebesar 2074 gL 3629 gL 3239 gL 2745 gL dan 4001 gL. IL dapat dilakukan recycle dan digunakan kembali dengan kemampuan melarutkan selulosa yang tidak berbeda jauh dibandingkan dengan fresh IL. Yield yang diperoleh dari sabut kelapa hasil pretreatment dengan IL recycle adalah sebesar 1455 recycle pertama dan 1451 recycle kedua. Yield sabut kelapa yang dilakukan pretreatment oleh NaOH lalu dilanjutkan pretreatment oleh IL recycle adalah 1892 recycle pertama dan 1846 recycle kedua. Kinerja IL recycle yang sangat baik ini merupakan hal yang sangat menguntukan dalam segi ekonomis.


ABSTRACT

The worldwide major problem for this recent century not also the increasing of green house gasses and pollutant in the atmosphere which was produce by over using the fossil fuel such as crude oil and natural gas but also the fossil fuel is nonrenewable energy source which means that its quantity is limited. To answer the problem a new source of energy must be found. The raw materials used to product reduction sugar is coconut coir. The chemical composition of coconut coir consist of 27 cellulosa 18 hemicellulosa 41 lignin 2 ash and 13 extract. The aim of this research is to get the best method in pretreatment coconut coir using the variables NaOH 1 liquid and NaOH 4 liquid IL Ionic Liquid or NaOH liquid and then advanced by IL. The method used consists of two steps which are pretreatment of raw materials both mechanically and chemically then hydrolysis of coconut coir to glucose by the cellulase enzyme. For the first step mechanical pretreatment coconut coir milled and sieved to obtain 115-120 mesh for the chemical pretreatment used 1 wt NaOH solution T 80 C and t 16 hour and then using ionic liquid solution at T 120 C for 15 hours. Enzymes used are pure cellulose enzyme from Aspergillus niger and xylanse enzyme from Trichoderma longibrachiatum which has been diluted with concentration 18.6 U grams of coir. Hydrolysis is used to convert cellulose into glucose and xylan into xylosa at temperature 60 C and pH 3.After pretreatment process be done the highest cellulosa be contains is 4333 coconut coir pretreatment product by NaOH 1 and then be continued by pretreatment IL. The highest hemicellulosa contains is 2518 derived from IL pretreatment and the lowest lignin derived from NaOH 1 pretreatment of coconut coir is 2522. Cellulosa and hemicellulosa producted by NaOH 1 pretreatment is more better than NaOH 4 pretreatment that is 4318 and 2057. Hydrolysis used mixed cellulase and xylanase enzyme product reduction sugar that is 3139 by the way hydrolysis using cellulase yield the highest product 2705 gram reduction sugar gram cellulosa hemicellulosapreliminary. Hydrolysis using mixed enzymes cellulase and xylanase product reduction sugar without pretreatment pretreatment NaOH 1 pretreatment NaOH 4 pretreatment IL and pretreatment NaOH combined by IL is 272 gL 3834 gL 3475 gL 3168 gL and 4645 gL. Hydrolysis using cellulase enzyme products reduction sugar for without pretreatment pretreatment NaOH 1 pretreatment NaOH 4 pretreatment IL and pretreatment NaOH 1 combined IL is 2074 gL 3629 gL 3239 gL 2745 gL and 4001 gL. IL can be done by recycle and be used again by the power to solute cellulose not too different be compared by fresh IL. Yield derived from coconut coir pretreatment product by IL recycle is 1455 for first recycle and 1451 for second recycle. Yield derived from coconut coir done by pretreatment of NaOH and then be combined or be advanced by IL pretreatment of recycle is 1892 for first recycle and 1846 for second recycle. This remuneration of IL recycle that so good is the one that so advantage of the economic side or the economic point of view.



KeywordsGula reduksi; Hemiselulosa; Hidrolisa; Ionic liquid; NaOH; Recycle; Debu Sabut Kelapa; Selulosa
 
Subject:  Gula
Contributor
  1. Prof. DR. Ir. Arief Widjaja, M. Eng
Date Create: 01/01/2015
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-23101140005898
Collection ID: 23101140005898
Call Number: RSK 661.802 Dew p


Source
Undergraduate Thesis of Chemical Engineering, RSK 661.802 Dew p, 2014

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