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ITS » Undergraduate Theses » Biologi
Posted by aguss at 06/06/2009 21:23:29  •  5700 Views


A PRELIMINARY TEST OF Spongosorites sp. EXTRACT FROM PASIR PUTIH SITUBONDO AS AN ANTIBACTERIAL

Author :
NURSAADAH, AFIFA 




ABSTRAK

Telah dilakukan uji pendahuluan aktivitas antibakteri pada Spongosorites sp. dari pantai pasir putih Situbondo terhadap bakteri Staphylococcus aureus. Spons diekstraksi dengan pelarut polar metanol dan non polar klorofom. Pengujian aktivitas antibakteri digunakan metode lubang agar Well diffusion method dengan antibiotik eritromisin sebagai kontrol positif. Konsentrasi ekstrak spons polar dan non polar yang digunakan adalah 0 ppm 5 ppm 10 ppm 15 ppm dan 20 ppm. Hasil menunjukkan besar zona terang antara 4 12 mm. Berdasarkan klasifikasi sensitifitas Kirby-Bauer termasuk kategori menghambat sedang. Selanjutnya dilakukan peningkatan konsentrasi ekstrak spons polar dan non polar menjadi 500 1000 1500 dan 2000 ppm. Hasil menunjukkan besar zona terang lebih dari 12 mm. Berdasarkan klasifikasi sensitifitas Kirby-Bauer termasuk kategori menghambat kuat. Hasil uji kualitatif pada ekstrak polar spons terdapat senyawa golongan alkaloid sedangkan pada ekstrak non polar spons terdapat senyawa golongan alkaloid dan terpenoid.


ABSTRACT

A preliminary test of antibacterial activities of Spongosorites sp. against Staphylococcus aureus has been performed. The sampel of sponge was taken from Pasir Putih Situbondo. The sponge was extracted with polar methanol and non polar solvent chlorofom. The well diffusion method was used for antibacterial activity test with antibiotic erithromicin as a positif control. The concentration of the extract were applied are 0 ppm 5 ppm 10 ppm 15 ppm and 20 ppm. The result showed that the clear zone was 4 12 mm. Based on the level sensitivities of bacteria by Kirby-Bauer 2007 the result demonstrated to intermediary inhibition. The concentration of the extract was increased into 500 ppm 1000 ppm 1500 ppm and 2000 ppm. More over the result showed that the clear zone increase more than 12 mm. Based on the level sensitivities of bacteria Kirby-Bauer it categorize to strong inhibition. The result of qualitative test of extract compounds showed that in polar extract was contained alkaloid whereas in non polar extract was contained terpenoid and alkaloid.



Keywordsaktivitas antibakteri ; Spongosorites sp ; Staphylococcus aureus ; metode difusi lubang agar
 
Subject:  Bakteriologi
Contributor
  1. Awik Puji Dyah N., S.Si., M.Si
    N.D. Kuswytasari, S.Si., M.Si
Date Create: 06/06/2009
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-3100009034304
Collection ID: 3100009034304
Call Number: RSBi 579.353 Nur u


Source
Undergraduate Theses, Biology, RSBi 579.353 Nur u, 2008

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