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ITS » Undergraduate Theses » Teknik Kimia
Posted by dee@its.ac.id at 26/10/2011 09:30:32  •  1298 Views


IMMOBILISASI ENZIM GODHRP UNTUK APLIKASI BIOSENSOR DENGAN METODE SOL GEL

THE IMMOBILIZATION OF GODHRP ENZYME FOR BIOSENSOR APLYCATION WITH SOL-GEL METHOD

Author :
UTAMI, WAHYU DHINI SISWI  ( 2306100009 )
FEBRIANA, YENY




ABSTRAK

Penelitian ini bertujuan untuk mempelajari pengaruh kondisi operasi pembuatan sol-gel seperti konsentrasi silika dalam sol terhadap porositas lapisan sol gel silika serta mempelajari pengaruh porositas suhu dan pH terhadap aktivitas dan stabilitas enzim yang di immobilisasi. Prosedur immobilisasi enzim kedalam silica gel terdiri dari dua tahap pertama adalah tahap pembuatan sol dengan menghidrolisa silicon alkoksida dalam air demin dengan pelarut etanol yang dikatalisa oleh asam dengan penambahan HCl. Reaksi hidrolisa dilakukan selama 61617 15 jam dan larutan dievaporasi untuk menghilangkan etanol. Tahap kedua adalah tahap pembuatan gel dan immobilisasi enzim. Larutan buffer pH 7 ditambahkan ke dalam sol dengan jumlah yang divariasi untuk memperoleh berbagai konsentrasi silika dari 131 sampai 525. Setelah itu enzim Glucose OxidaseGOD dan Horse radish PeroxidaseHRP yang juga dilarutkan ke dalam buffer pH 7 ditambahkan ke dalam sol. Larutan sediaan yang mengandung kedua enzim tersebut kemudian dilapiskan pada plat kaca untuk membentuk lapisan yang tipis. Luas permukaan dan diameter pori lapisan matriks silika diukur dengan metoda adsorpsidesorpsi nitrogen. Aktivitas enzim terimmobilisasi diuji dengan prosedur Sigma Protokol. Dengan metode adsorpsidesorpsi nitrogen didapatkan bahwa semakin besar konsentrasi silika dalam larutan sol maka semakin besar luas permukaan dan semakin kecil diameter pori. Dari analisa spektrofotometer UV-vis didapatkan hasil aktivitas enzim yang terimmobilisasi paling bagus4361 unitml pada konsentrasi 15 W SiO2 luas permukaan0387 m2g diameter pori2896. Untuk stabilitas enzim terimmobilisasi paling bagus ketika ditreatment pada suhu 2561616C 3696 dan pH 6 1706.


ABSTRACT

The main objective of this research is to study the influence of the operating conditions making sol-gel such as silica concentration in the sol for silica sol-gel layer porosity and to study the influence of porosity temperature and pH for activity and stability in the immobilization of enzymes. The procedure of enzyme immobilization in Silica gel is consists of two steps. The first step is preparing the sol solution with hydrolize silicon alkoxide in water demin with ethanol which it catalyticed by the addition of HCl. The hydrolysis reaction carried out for 6161715 hours and the solution evaporated to remove ethanol. The second steps is preparing the gel and the immobilization of enzymes. PH 7 buffer solution added to the sol so that can give the variety concentrations of silica from 1.31 to 5.25. After that the enzyme Glucose oxidase GOD and Horse Radish Peroxiduse HRP which its also dissolved into pH 7 buffer added to the sol. Stock solution that contains both the enzyme is coated on a glass plate to form a thin layer. Surface area and pore diameter of the silica matrix layer is measured by the method of adsorption desorption of nitrogen. the activity of immobilization enzyme tested by Sigma Protocol procedures. With the method of adsorption desorption nitrogen showed that the higher concentration of silica in the solution sol the greater the surface area and the smaller the pore diameter. From the analysis of UV-vis spectrophotometer the result shows that the best activity of immobilization enzyme 4.361 units ml at a concentration of 1.5 W SiO2 surface area 0.387 m2 g pore diameter 2896. For the stability of immobilization enzyme when treated at a temperature 2561616C36.96gandbpHm617.06.



KeywordsImmobilisasi Enzim; Glucose oxidase; Horse Radish Peroxidase; Sol-Gel
 
Subject:  Immobilized enzymes
Contributor
  1. Prof. DR. Ir. Heru Setyawan, M.Eng
Date Create: 26/07/2010
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-3100011043420
Collection ID: 3100011043420
Call Number: RSK 660.634 Wah i


Source
Undergraduate Thesis of Chemical Engineering, RSK 660.634 Wah i, 2010

Coverage
ITS Community

Rights
Copyright @2010 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




[ Download - Open Access ]

  1.  ITS-Undergraduate-15553-2306100009-Abstract_id.pdf - 89 KB
  2.  ITS-Undergraduate-15553-2306100009-Abstract_en.pdf - 90 KB
  3.  ITS-Undergraduate-15553-2306100009-Conclusion.pdf - 91 KB
  4.  ITS-Undergraduate-15553-2306100009-Paper.pdf - 290 KB




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