EMAIL: PASSWORD:
Front Office
UPT. PERPUSTAKAAN
Institut Teknologi Sepuluh Nopember Surabaya


Kampus ITS Sukolilo - Surabaya 60111

Phone : 031-5921733 , 5923623
Fax : 031-5937774
E-mail : libits@its.ac.id
Website : http://library.its.ac.id

Support (Customer Service) :
timit_perpus@its.ac.id




Welcome..guys!

Have a problem with your access?
Please, contact our technical support below:
LIVE SUPPORT


Moh. Fandika Aqsa


Davi Wahyuni


Tondo Indra Nyata


Anis Wulandari


Ansi Aflacha




ITS » Paper and Presentation » Biologi
Posted by fandikaaqsa@its.ac.id at 28/04/2016 09:27:49  •  521 Views


AZOTOBACTER AS A MERCURY BIOACCUMULATOR

Azotobacter SEBAGAI BIOAKUMULATOR MERKURI

Author :
KHOTIMAH, KUSNUL ( 1510100043 )




ABSTRAK

Merkuri merupakan logam berat paling toksik dibandingkan dengan logam berat lainnya. Beberapa bakteri ada yang resisten merkuri. Salah satu genus bakteri resisten merkuri dan mampu mengakumulasi merkuri yaitu Azotobacter. Azotobacter merupakan bakteri pemfiksasi nitrogen bebas non simbiotik yang melimpah di daerah rhizosfer lahan pertanian dan merupakan bakteri penghasil EPS yang dapat berfungsi sebagai pengkhelat logam. Penelitian ini bertujuan untuk mendapatkan isolat Azotobacter yang resisten terhadap merkuri HgCl2 dan mengukur kemampuan bioakumulasinya terhadap HgCl2. Isolasi bakteri Azotobacter dilakukan dengan media selektif Azotobacter. uji resistensi HgCl2 dilakukan dengan streak agar miring dan kemampuan bioakumulasi diukur dengan metode serapan atom serta uji viabilitas menggunakan metode pour plate. Analisis beda nyata dengan ANOVA pada taraf 5 dilanjutkan dengan uji beda nyata terkecil BNT. Tiga isolat Azotobacter dari lahan eco urban farming ITS resisten terhadap HgCl2 sampai 20 mgL yaitu A5 A6 dan A9. Efisiensi bioakumulasi yang tertinggi pada pemaparan HgCl2 5 mgL yaitu isolat A5 89 dan A9 87.


ABSTRACT

Mercury is the most toxic heavy metals compared with other heavy metals. Some bacteria are resistant to mercury. One of mercury resistant bacteria genus and able to accumulate of mercury is Azotobacter. Azotobacter is free-living nitrogen fixing bacteria which abundant in rhizosfer area of agricultural land and it is EPS producing bacteria that can serve as chelating metals. This research aims to get Azotobacter isolates resistant to mercury HgCl2 and measure the ability of mercury biaccumulation. Isolation of Azotobacter by selective Azotobacter media resistance test by streak at slant agar and bioaccumulation ability measured by atomic absorption method and vaibility test using pour plate method. Observed data analyzed by ANOVA continued by Least Significant Difference LSD test Both test at level 5 . Three Azotobacter isolates from eco urban farming ITS land are resistant until 20 mgL of HgCl2 which is A5 A6 and A9. The highest efficiency bioaccumlation on exposure 5 mgL HgCl2 is A5 89 and A9 87.



KeywordsAzotobacter; bioakumulator; merkuri; uji resistensi
 
Subject:  Bioteknologi
Contributor
  1. Dr. Enny Zulaika, M.P196001091988032001
Date Create: 28/04/2016
Type: Text
Format: PDF
Language: Indonesian
Identifier: ITS-paper-15021160008836
Collection ID: 15021160008836
Call Number: RSBi 571.954 663 Kho a


Source
Paper and Presentation of Biology, RSBi 571.954 663 Kho a, 2014

Coverage
ITS Community

Rights
Copyright @2016 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




[ Download - Open Access ]

  1.  ITS-paper-41380-1510100043-Paper.pdf - 251 KB
  2.  ITS-paper-41380-1510100043-Presentation.pdf - 1145 KB




 Similar Document...




! ATTENTION !

To facilitate the activation process, please fill out the member application form correctly and completely

Registration activation of our members will process up to max 24 hours (confirm by email). Please wait patiently

POLLING

Bagaimana pendapat Anda tentang layanan repository kami ?

Bagus Sekali
Baik
Biasa
Jelek
Mengecewakan





You are connected from 54.198.28.114
using CCBot/2.0 (http://commoncrawl.org/faq/)



Copyright © ITS Library 2006 - 2017 - All rights reserved.
Dublin Core Metadata Initiative and OpenArchives Compatible
Developed by Hassan