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ITS » Paper and Presentation » Biologi
Posted by aprill@is.its.ac.id at 17/02/2016 13:59:49  •  824 Views


THE INFLUENCE OF ORGANIC WASTE MEDIUM AGAINST THE ACTIVITY OF PROTEASE ENZYME FROM WON0REJO MOLDS ISOLATE

PENGARUH MEDIUM LIMBAH ORGANIK TERHADAP AKTIVITAS ENZIM PROTEASE DARI ISOLAT KAPANG TANAH WONOREJO

Author :
DEWI, NI WAYAN NANIK SANTIKA ( 1510100021 )




ABSTRAK

Meningkatnya akumulasi limbah cair organik yang mengandung protein di lingkungan perlu ditanggulangi. Penelitian ini bertujuan untuk mengetahui aktivitas enzim protease dari Penicillium sp. 3 T3F2 Verticillium sp. T3.3 dan Trichoderma sp. 1 T3I2 secara tunggal dan konsorsium pada medium limbah cair organik. Parameter yang diuji dalam penelitian ini adalah aktivitas enzim protease dan degradasi protein isolat pada medium limbah cair pencucian ikan limbah tangki septik dan limbah cair tahu. Data pengaruh medium terhadap aktivitas enzim protease dianalisis menggunakan Annova One-Way dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa isolat berpengaruh terhadap aktivitas enzim protease dari ketiga medium. Konsorsium isolat Penicillium sp. 3 T3F2 Verticillium sp. T3.3 memiliki nilai aktivitas enzim protease dan degradasi protein tertinggi pada ketiga jenis medium. Pada medium limbah cair pencucian ikan 0.219 UmL 48.394 . Pada medium limbah tangki septik 0.149 UmL 31.268 . Pada limbah cair tahu 0.222 UmL 44.627 .


ABSTRACT

Increasing the accumulation of organic wastewater containing proteins in the neighborhood need to overcome. This research is to know the activity of an enzyme protease from Penicillium sp. 3 T3F2 Verticillium sp. T3.3 and Trichoderma sp. 1 T3I2 single and a consortium on substrate organic wastewater. The parameters tested in this research is activity of an enzyme protease and degradation of protein by isolates on substrate fish processing wastewater septic tank wastewater and tofu wastewater. The influence data of substrate against the activity of an enzyme protease analyzed using Annova One-Way and continued with Duncan test. The result showed that substrate and isolates affect the activity of enzymes protease. A consortium of isolates Penicillium sp. 3 T3F2 Verticillium sp.T3.3 having value activity of an enzymes protease and degradation of the protein highest in the three types of substrate. In substrate fish processing wastewater 0.219 UmL 48.394 . In Septic tank wastewater 0.149 UmL 31.268 . In tofu wastewater 0.222 UmL 44.627.



KeywordsLimbah Organik; Protease; Degradasi Protein
 
Subject:  Lipase - bioteknologi
Contributor
  1. Nengah Dwianita Kuswytasari, S.Si.,M.Si
Date Create: 17/02/2016
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-paper-15121150008368
Collection ID: 15121150008368
Call Number: RSBi 572.76 Dew p


Source
Paper and Presentations, Biology, RSBi 572.76 Dew p, 2016

Coverage
ITS Community

Rights
Copyright @2016 by ITS Library. This publication is protected by copyright and per obtained from the ITS Library prior to any prohibited reproduction, storage in a re transmission in any form or by any means, electronic, mechanical, photocopying, reco For information regarding permission(s), write to ITS Library




[ Download - Open Access ]

  1.  ITS-paper-40174-1510100021-paper.pdf - 446 KB
  2.  ITS-paper-40174-1510100021-presentation.pdf - 1450 KB




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