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ITS » Master Theses » Teknologi Proses - S2
Posted by eny at 05/01/2007 17:45:26  •  13639 Views


PRODUKSI ENZIM XYLANASE DARI ASPERGILLUS NIGER ATCC 6275 PADA MEDIA FERMENTASI TERENDAM DENGAN SUBSTRAT DEDAKGANDUM

Production Of Xylanase From Aspergillus niger ATCC 6275 Under Submerged Fermentation With Wheat Bran as Substrate

Created by :
SANDJAJA, ANTONIUS RONNY 



SubjectFermentasi
KeywordXylanase
Aspergillus niger
ATCC 6275
Fermentation
Wheat Bran

Description:

Produksi enzim telah dilakukan melalui media fermentasi terendam dengan menggunakan jamur Aspergillus niger di dalam medium fermentasi yang mengandung dedak gandum sebagai substrat. Enzim yang dihasilkan antara lain enzim xylanase dalam jumlah besar dengan sedikit enzim selulase. Aspergillus niger ATCC 6275 di kultivasikan dalam erlenmeyer yang berisi substrat dedak gandum dan larutan medium fermentasi yang mengandung garam-garam mineral dan sumber nitrogen pada fermentasi terendam. Tujuan penelitian ini adalah menghasilkan enzim xylanase dengan aktivitas tinggi. Variabel yang digunakan adalah perbedaan konsentrasi substrat 1%,2%,3%,4% , jumlah spora Ixl05-lxl06 ; lxl06-lxl07 ; 1xl07-lxl08 spora/ml, medium fermentasi I dan II dan juga waktu inkubasi 2,3,4,5 dan 6 hari. Dari hasil percobaan menunjukkan bahwa proses fermentasi terendam dengan medium larutan garam-garam mineral dan sumber nitrogen, diperoleh aktivitas enzim xylanase terbesar adalah 25,59 IU/ml pada konsentrasi substrat 1% jumlah spora lxl07-lxl08 spora/ml dan waktu inkubasi 4 hari dengan konsentrasi protein yang terbentuk 0,8122 mg/ml. Enzim xylanase mempunyai aktivitas terbesar pada suhu 50 C dan pH 5. Purifikasi enzim dengan presipitasi ammonium sulfat menghasilkan aktifitas 36,75 IU/ml. Sedangkan aktivitas enzim selulase 2,35 IU/ml dihasilkan pada konsentrasi substrat 1% jumlah spora Ixl07-lxl08 spora/ml dan waktu inkubasi 3 hari. Pada aplikasi enzim terhadap pulp didapatkan persentase degradasi terbesar terhadap hemiselulosa, selulosa dan lignin pada waktu reaksi 12 jam masing-masing sebesar 24,69%, selulosa 8,60 % dan lignin 1,61%.


Alt. Description

Enzyme was produced under submerged fermentation by using Aspergillus niger fungi in the fermentation medium containing wheat bran as substrate. The xylanase enzyme has been produced from Aspergillus niger with a little amounts of cellulase. Aspergillus niger ATCC 6275 were cultivated in erlenmeyer containing wheat bran as substrate and fermentation medium containing minerals salt solutions and nitrogen source under submerged fermentation. The objective of the research is producing xylanase enzyme with high activity. The variable of this research was substrate concentration 1%, 2%, 3%, and 4%, spora concentration Ixl05-lxl06 ; lxl06-lxl 07; lxl07-lxl08 spora/ml, type of fermentation medium I and II and incubation time 2,3,4,5 and 6 days. The experiment result showed that submerged fermentation process with medium containing mineral salt solution and nitrogen source gives the highest xylanase enzyme activity of 25,59 IU/ml on 1% substrate concentration, spora concentration Ixl07-lxl08 spora/ml and 4 days incubation with protein concentration of 0,8122 mg/ml. Xylanase enzyme gives the highest activity at 50°C and pH 5. Enzyme purification with ammonium sulphate precipitation gives xylanase activity of 36,75 IU/ml. Cellulase enzyme with an activity of 2,35 IU/ml was produced on 1% substrate concentration, spora concentration 1x10 -1x10 spora/ml and 3 days incubation. The enzyme application into pulp gives the highest degradation of hemicellulose, cellulose and lignin at 12 hours reaction, that is 24,69%, 8,60% and 1,61% respectively.

Contributor:
  1. Dr. Ir. Arief Widjaja, M.Eng
Date Create:05/04/2005
Type:Text
Format:pdf ; 151 pages
Language:Indonesian
Identifier:ITS-Master-3100005023229
Collection ID:3100005023229
Call Number:572.7 San p


Source :
Theses Chemical Engineering RTK 572.7 San p, 2005

Coverage :
ITS Community

Rights :
Copyright @2005 by ITS Library. This publication is protected by copyright and permission should be obtained from the ITS Library prior to any prohibited reproduction, storage in a retrievel system, or transmission in any form or by any means, electronic, mechanical, photocopying, recording, or likewise. For information regarding permission(s), write to ITS Library


Publication URL :
http://digilib.its.ac.id/produksi-enzim-xylanase-dari-aspergillus-niger-atcc-6275-pada-media-fermentasi-terendam-dengan-substrat-dedakgandum-705.html




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6275 , ATCC , ATCC 6275 , Aspergillus , Aspergillus niger , Bran , Fermentation , Wheat , Wheat Bran , Xylanase , niger



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