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ITS » Undergraduate Theses » Kimia
Posted by aseri at 22/01/2009 16:26:13  •  4909 Views


ANALYSIS CONTENT OF HOPANOIDS FROM PLANTATION SOIL IN SAMBIROTO NGANJUK

Author :
WIDIYANINGSIH, ENDAH  




ABSTRAK

Bakteri merupakan kelompok mikroorganisme paling dominan dalam tanah. Salah satu bakteri tanah adalah bakteri penyerap nitrogen yang mengandung hopanoid. Hopanoid merupakan senyawa metabolit sekunder golongan terpena. Analisis hopanoid pada tanah dapat menunjukkan adanya korelasi antara kandungan hopanoid dengan jenis tanah. Penelitian ini mengambil cuplikan tanah dari perkebunan desa Sambiroto Nganjuk pada kedalaman 30 cm.Kemudian diekstrak dengan metode sokhletasi menggunakan pelarut kloroformmetanol 21. Ekstrak lipid tanah yang diperoleh kemudian didegradasi dengan metode H5IO6NaBH4. Analisis kromatografi gas spektroskopi massa KG SM digunakan untuk menentukan jenis hopanoid dan asam lemak dari fraksi hopanoid yang terdapat dalam tanah. Akan tetapi spektrum massa dari penelitian menunjukkan fragmen spesifik hopanoid mz 191 dan 263 dengan kelimpahan yang relatif kecil sehingga kemungkinan hopanoid dalam tanah tersebut merupakan senyawa minor. Senyawa yang terdeteksi adalah asam lemak yaitu etil palmitat 0005 mgg cuplikan tanah dan etil cerotat 0018 mgg cuplikan tanah.


ABSTRACT

Bacteria are the most dominant microorganism in soil.Nitrogen fixing bacteria are one of soil bacteria which consist of hopanoids. Hopanoids are secondary metabolit compound of terpena. Analysis of hopanoids can be illustrated by correlation between hopanoids and soil invironments. This research takes some of sample from plantation in Sambiroto Nganjuk depth about 30 centimetres. It is extracted by sokhletation method with solvent of chloroformmethanol 21. Then lipid extracts in soil are degradated by H5IO6NaBH4 method. In Gas Chromatography-Mass Spectroscopy GC-MS is used to determine kind of hopanoids and fatty acid of hopanoids fraction containing in soil. But mass spectra from this research indicate that spesific fragment of hopanoids mz 191 and 263 with small quantity so it is possible that hopanoids in plantation soil is minor compound. The compounds which detected are fatty acids consist of ethyl palmitic 0005 mgg sample and ethyl cerotic 0018 mgg sample.



KeywordsHopanoid ; Asam lemak ; KG – SM
 
Subject:  Metabolisme
Contributor
  1. DR. Surya Rosa Putra, MS
Date Create: 22/01/2009
Type: Text
Format: pdf
Language: Indonesian
Identifier: ITS-Undergraduate-3100008032646
Collection ID: 3100008032646
Call Number: RSKi 572.84 Wid a


Source
Undergraduate thesis, Chemistry Departement, RSKi 572.84 Wid a, 2008

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